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Palapala Kahe

Hōʻike

kikoʻī

ʻO Cordyceps militaris kahi ʻano lāʻau lapaʻau kūʻokoʻa a koʻikoʻi i ka Chinese Cordyceps, i hoʻohana nui ʻia ma ke ʻano he biocontrol agents ma Kina no nā kenekulia.

Ua hoʻokaʻawale maikaʻi ʻia ʻo Cordycepin mai Cordyceps militaris me ka hoʻohana ʻana i ka hoʻoheheʻe ʻana me ka wai ma lalo o kahi mahana, a i ʻole ka hui ʻana o ka ethanol a me ka wai. Hoʻoholo ʻia ka mahana kūpono loa, ka wai a i ʻole ka hui ʻana o ka ethanol i ka wai, ka lākiō solvent/solid a me ka pH o ka mea hoʻoheheʻe e pili ana i ka loaʻa ʻana. ʻO ka hua kiʻekiʻe loa no ka cordycepin (90% +) i wānana ʻia e ka regression model a hōʻoia ʻia e ka hoʻohālikelike ʻana me nā hopena hoʻokolohua, e hōʻike ana i ka ʻaelike maikaʻi. Ua hoʻohana ʻia ke ʻano RP-HPLC no ka nānā ʻana i ka cordycepin mai nā ʻāpana Cordyceps militaris, a ua loaʻa ka 100% maʻemaʻe o ka cordycepin. Ua noiʻi ʻia nā hiʻohiʻona o ka unuhi ʻana ma ke ʻano o ka equilibrium a me nā kinetics.

ʻO kekahi mau ʻōlelo aʻoaʻo e pili ana i ka ʻokoʻa ma waena o CS-4 a me Cordyceps sinensis a me Cordyceps militaris

1. CS-4 'o ia no ka cordyceps sinensis number 4 fungal strain —-Paecilomyces hepiali — he ha'o endoparasitic keia e noho mau ana i ka cordyceps sinensis maoli.

2. Ua hoʻokaʻawale ʻia ʻo Paecilomyces hepiali mai ka cordyceps sinensis maoli, a ua hoʻopaʻa ʻia ma luna o nā substrate hana (paʻa a wai paha) e ulu ai. He hana kēia o ka fermentation. paʻa paʻa —solid status fermentation (SSF), liquid substrate—Submerged fermentation (SMF).

3. I kēia manawa ʻo Cordyceps militaris wale nō (ʻo ia kekahi ʻano o ka cordyceps) ʻo ka mycelium a me ke kino hua i loaʻa i ka cordycepin.  a aia kekahi ʻano o nā cordyceps (Hirsutella sinensis), a he cordycepin pū kekahi. Akā ʻo Hirsutella sinensis wale nō ka mycelium i loaʻa.