Zovala zapamwamba za ODM

Tchati Choyenda

Kufotokozera

Tsatanetsatane

Cordyceps militaris ndi mafangasi apadera komanso amtengo wapatali azachipatala ku China Cordyceps, omwe akhala akugwiritsidwa ntchito ngati othandizira ku China kwazaka zambiri.

Cordycepin idalekanitsidwa bwino ndi ankhondo a Cordyceps pogwiritsa ntchito kutulutsa ndi madzi pokhapokha kutentha kwina, kapena kusakaniza kwa ethanol ndi madzi. Kutentha kokwanira, madzi kapena kapangidwe ka ethanol m'madzi, chiŵerengero cha zosungunulira / zolimba ndi pH ya zosungunulira zinatsimikiziridwa ponena za zokolola za m'zigawo. Zokolola zapamwamba kwambiri za cordycepin (90% +) zinanenedweratu ndi chitsanzo chochepetsera ndikutsimikiziridwa poyerekeza ndi zotsatira zoyesera, kusonyeza kuvomerezana kwabwino. Njira ya RP-HPLC idagwiritsidwa ntchito kusanthula cordycepin kuchokera ku Cordyceps militaris extracts, ndipo 100% yoyera ya cordycepin idakwaniritsidwa. Makhalidwe a m'zigawo adafufuzidwa malinga ndi kufanana ndi kinetics.

Malangizo ena okhudza kusiyana kwa CS-4 ndi Cordyceps sinensis ndi Cordyceps militaris

1. CS-4 imayimira cordyceps sinensis number 4 fungal strain —-Paecilomyces hepiali — uwu ndi mafangasi omwe amakhala m'matumbo achilengedwe a cordyceps sinensis.

2. Paecilomyces hepiali anasiyanitsidwa ndi chilengedwe cordyceps sinensis, ndipo intemera pa opangira magawo (olimba kapena madzi) kukula. Iyi ndi njira yowotchera. solid substrate —solid status fermentation (SSF), liquid substrate—Submerged fermentation (SMF).

3. Mpaka pano kokha cordyceps militaris (uwu ndi mtundu wina wa cordyceps) 's mycelium ndi fruiting body has cordycepin.  ndipo palinso mtundu wina wa cordyceps ( Hirsutella sinensis ), ulinso ndi cordycepin. Koma Hirsutella sinensis ndi mycelium yokha yomwe ilipo.