Cordycepin, kana 3′-deoxyadenosine, inobva panucleoside adenosine. Iyo bioactive komisheni inogona kutorwa kubva kumarudzi akasiyana eCordyceps fungus, kusanganisira Cordyceps militaris uye Hirsutella sinensis (an artificial fermentation mycelium ye ophiocordyceps sinensis).
Izvo zvinofanirwa kutaurwa zvakanyanya ndezvekuti bvunzo dzinoratidza muviri unobereka weatual ophiocordyceps sinensis haina cordycepin, asi ine yakakwira zvemukati mesimbi inorema, kunyanya Arsenic.
Maitiro ekubvisa cordycepin anogona kuitwa kuburikidza nematanho anotevera:
1. Kusarudzwa kwezvipenyu zvefungal: Danho rokutanga nderokusarudza mhando dzakakodzera dzeCordyceps fungus kuti dzibvise. Cordyceps militaris inowanzofarirwa nekuti ine mazinga epamusoro ecordycepin kupfuura mamwe marudzi. Hirutella inodhura zvakanyanya kuita yekubvisa. Saka cordyceps militaris ndiyo yekutanga sarudzo kusvika zvino.
2.Kurimwa kwefungus: Iyo cordyceps militaris inorimwa munzvimbo inodzorwa kuti ive nechokwadi chekukura uye cordycepin kugadzirwa. Izvi zvinogona kusanganisira kukura fungus pane substrate, senge mupunga kana soya bhinzi, pasi pemamiriro chaiwo etembiricha, hunyoro, uye chiedza.
Isu tinowanzo sarudza izvo zvekutanga zve cordycepin muhuwandu hwe0.1-0.3% (inoda mamwe ma substrates, mupunga uye soya bean poda). Kazhinji, iyo cordyceps militaris neiyo substrates yegorosi bran chete ine 0.05% cordycepin kana kunyange zvishoma.
3.Kukohwa nekuomesa: Kana fungus yasvika pakukura, inokohwewa uye yakaomeswa kubvisa unyoro hwakawandisa.
4. Kubviswa kwe cordycepin: Iyo yakaomeswa fungal zvinhu zvino inogayiwa kuita poda yakatsetseka uye inoiswa pasi pekubvisa uchishandisa yakakodzera solvent. Cordycepin inogona kunyungudika mumvura uye ethanol solution.Isu tinowanzo shandisa mvura yekubvisa nekuti inoratidza zvirinani kuita mutengo uye nyore kudzora.
Iyo poindi yekubvisa mvura kuti uwane cordycepin ndeye kudzora tembiricha pasi pemutengo wakatarwa, kazhinji pasi pe70 degree centigrade. Zvikasadaro, ichava hydrolyzed nyore.
5.Kucheneswa: Iyo inoguma yakasvibiswa yakasvibiswa inobva yacheneswa uchishandisa nzira dzakasiyana-siyana dzakadai sechromatography, precipitation, kana crystallization kuparadzanisa cordycepin compound.
Munzvimbo yedu, tinoshandisa chromatography(cationic resin) kugadzira yakakwira yecordycepin kubva pa5% kusvika 95% (iyi ndiyo nhamba yepamusoro yataita kusvika parizvino)
Kazhinji, cordycepin kubva ku0.5% - 3% haifanirwe kucheneswa.
6.Kuongorora uye kuedza: Chigadzirwa chekupedzisira chinoongororwa uye chakaedzwa kuchena, potency, uye hutano kuti ive nechokwadi chokuti inosangana nezvinodiwa.
Saka zvakazara, maitiro ekubvisa anosanganisira: Kudhirowa kweMvura, kusefa, Concentration, Kucheneswa, Kuomesa, Sieving, uye Kuonekwa kweSimbi.
Kuedzwa kwe cordycepin kwakanyatso kusimbiswa. Muchidimbu, kushandisa HPLC nereferensi sampuli yeCordycepin. Makoramu anonyanya kushandiswa kupatsanura cordycepin iC18 columns ine particle size ye 3-5 µm uye kureba 150-250 mm. Titumire email kana uchida kuziva zvakawanda nezvazvo.
Chimwezve chinhu, iyo substrates mushure me cordyceps militaris 'fruiting body inokohwewa zvakare ine diki nhamba yecordycepin. Saka kubviswa kwayo kunogona kuve ne 0.2 - 0.5% cordycepin.
Nguva yekutumira:May-16-2023