I-Odm ephezulu yeLew izinga lesikhumba

Ishadi Eligelezayo

Ukucaciswa

Imininingwane

I-Cordyceps militaris iyisikhunta sezokwelapha esiyingqayizivele nesiyigugu e-Chinese Cordyceps, esisetshenziswe kabanzi njengama-biocontrol e-China amakhulu eminyaka.

I-Cordycepin yahlukaniswa ngempumelelo kumasosha aseCordyceps kusetshenziswa isizinda ngamanzi ngaphansi kwezinga lokushisa elithile, noma ingxube ye-ethanol namanzi. Izinga lokushisa eliphezulu, amanzi noma ukwakheka kwe-ethanol emanzini, isilinganiso se-solvent/solid kanye ne-pH ye-solvent kwanqunywa ngokuphathelene nesivuno sokukhishwa. Isivuno esiphezulu se-cordycepin (90%+) sabikezelwa imodeli yokuhlehla futhi saqinisekiswa ngokuqhathanisa nemiphumela yokuhlola, okubonisa ukuvumelana okuhle. Indlela ye-RP-HPLC yasetshenziswa ukuze kuhlaziywe i-cordycepin evela ku-Cordyceps militaris ekhishwe, futhi ukuhlanzeka okungu-100% kwe-cordycepin kwafinyelelwa. Izici zokukhishwa zaphenywa ngokuya ngokulingana kanye ne-kinetics.

Amanye amathiphu mayelana nomehluko phakathi kwe-CS-4 ne-Cordyceps sinensis ne-Cordyceps militaris

1. I-CS-4 imele i-cordyceps sinensis number 4 fungal strain —-Paecilomyces hepiali — lesi ukhunta endoparasitic esivame ukuba khona ku-cordyceps sinensis yemvelo.

2. I-Paecilomyces hepiali yahlukaniswa ku-cordyceps sinensis yemvelo, futhi yajova kuma-substrates okwenziwa (okuqinile noma okuwuketshezi) ukuze ikhule. Lena inqubo yokuvutshelwa. i-solid substrate—i-solid status fermentation (SSF), i-substrate ewuketshezi—Ukuvutshelwa okungaphansi kwamanzi (SMF).

3. Kuze kube manje kuphela i-cordyceps militaris (lolu olunye uhlobo lwe-cordyceps) i-mycelium ne-fruiting body ine-cordycepin.  futhi kunolunye uhlobo lwecordyceps ( Hirsutella sinensis ) , nalo line - cordycepin . Kodwa i-Hirsutella sinensis itholakala kuphela nge-mycelium.