I-OEMP ye-OEL EBALASELULA EZIQHELEKILEYO-ISIQINISEKISO SESIFUNDO SEBENZISELWANO ZE-HERABROROM HOHROROROROROOM

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ICordyceps militaris sisingundo esikhethekileyo kwaye esixabisekileyo sonyango kwiCordyceps yaseTshayina, ebisetyenziswa ngokubanzi njengeearhente zolawulo lwebhayoloji eTshayina kangangeenkulungwane.

ICordycepin yahlulwa ngempumelelo kwi-Cordyceps militaris kusetyenziswa ukutsalwa kwamanzi kuphela phantsi kobushushu obuthile, okanye umxube we-ethanol namanzi. Ubushushu obuphezulu, amanzi okanye ukubunjwa kwe-ethanol emanzini, umlinganiselo we-solvent/solid kunye ne-pH ye-solvent iye yamiselwa ngokubhekiselele kwi-extraction isivuno. Isivuno esiphezulu se-cordycepin (90% +) saqikelelwa yimodeli yokubuyisela kwaye yaqinisekiswa ngokuthelekiswa neziphumo zovavanyo, ebonisa isivumelwano esihle. Indlela ye-RP-HPLC isetyenziswe ukuhlalutya i-cordycepin esuka kwi-Cordyceps militaris extracts, kunye ne-100% yokucoceka kwe-cordycepin yaphunyezwa. Iimpawu zokutsalwa ziye zaphandwa ngokweequilibrium kunye nekinetics.

Ezinye iingcebiso malunga nomahluko phakathi kwe-CS-4 kunye ne-Cordyceps sinensis kunye ne-Cordyceps militaris

1. I-CS-4 imele i-cordyceps sinensis inombolo ye-4 fungal strain --Paecilomyces hepiali - le yi-endoparasitic fungus edla ngokubakho kwi-natural cordyceps sinensis.

2. I-Paecilomyces hepiali yayihlulwe kwi-cordyceps sinensis yendalo, kwaye ifakwe kwi-substrates eyenziweyo (eqinile okanye engamanzi) ukuze ikhule. Le yinkqubo yokubilisa. i-solid substrate -i-solid status fermentation (SSF), i-liquid substrate-I-Fermentation fermentation (SMF).

3. Ukuza kuthi ga ngoku kuphela icordyceps militaris (olu lolunye uhlobo lwecordyceps) ‘s mycelium kunye nomzimba ovelisa iziqhamo unecordycepin.  kwaye kukho olunye uhlobo lwecordyceps ( Hirsutella sinensis ) , nayo inecordycepin . Kodwa i-Hirutella sinensis ifumaneka kuphela yi-mycelium.