I-OEM OLULA AMANQAKU IIMPAWU ZOKUGCINWA-ISIQINISEKISO SESIFUNDO SEBENZISELWANO ZE-HERBABROROROOM

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ICordyceps militaris yifungus yonyango ekhethekileyo kwaye ixabisekileyo kwiCordyceps yaseTshayina, ebisetyenziswa kakhulu njengeearhente zolawulo lwebhayoloji eTshayina kangangeenkulungwane.

ICordycepin yahlulwa ngempumelelo kwiCordyceps militaris kusetyenziswa ukutsalwa ngamanzi kuphela phantsi kobushushu obuthile, okanye umxube we-ethanol namanzi. Ubushushu obuphezulu, amanzi okanye ukubunjwa kwe-ethanol emanzini, umlinganiselo we-solvent/solid kunye ne-pH ye-solvent yamiselwa ngokubhekiselele kwisivuno sokukhutshwa. Isivuno esiphezulu se-cordycepin (90% +) saqikelelwa yimodeli yokubuyisela kwaye yaqinisekiswa ngokuthelekiswa neziphumo zovavanyo, ebonisa isivumelwano esihle. Indlela ye-RP-HPLC isetyenziswe ukuhlalutya i-cordycepin esuka kwi-Cordyceps militaris extracts, kunye ne-100% yokucoceka kwe-cordycepin yaphunyezwa. Iimpawu zokutsalwa ziye zaphandwa ngokweequilibrium kunye nekinetics.

Ezinye iingcebiso malunga nomahluko phakathi kwe-CS-4 kunye ne-Cordyceps sinensis kunye ne-Cordyceps militaris

1. I-CS-4 imele i-cordyceps sinensis inombolo ye-4 fungal strain --Paecilomyces hepiali - le yi-endoparasitic fungus edla ngokubakho kwi-natural cordyceps sinensis.

2. I-Paecilomyces hepiali yayihlulwe kwi-cordyceps sinensis yendalo, kwaye ifakwe kwi-substrates eyenziweyo (eqinile okanye engamanzi) ukuze ikhule. Le yinkqubo yokubilisa. i-solid substrate -i-solid status fermentation (SSF), i-liquid substrate-I-Fermentation fermentation (SMF).

3. Ukuza kuthi ga ngoku kuphela icordyceps militaris (olu lolunye uhlobo lwecordyceps) ‘s mycelium kunye nomzimba ovelisa iziqhamo unecordycepin.  kwaye kukho olunye uhlobo lwecordyceps ( Hirsutella sinensis ) , nayo inecordycepin . Kodwa i-Hirutella sinensis ifumaneka kuphela yi-mycelium.